I am currently a Graduate Research Assistant (GRA) in the department of Plant Pathology, Institute for Sustainable Food Systems - ISFS, and Emerging Pathogens Institute - EPI at the University of Florida. I am interested in understanding the ecology of microbial communities, mostly microbial assemblies, diversities, interactions, and their community level functions, in the natural as well as in the agricultural systems. My current work utilizes high-throughput amplicon sequencing and network models to understand the responses of rhizosphere microbial community in the grafted tomato systems.
Prior to joining the Garrett Lab at UF, I was a graduate student with Dr. Garrett at Kansas State University. I received my B.S. (with honors) in Biology from Truman State University. As an undergraduate student, I was very lucky to work in the lab of Dr. Jose Herrera. My undergraduate research projects were mostly related with understanding the ecology and distribution of root-associated fungal communities in the forage grasses and crops.
PhD in Microbial Metagenomcis and Network Modeling, 2013 - 2018
University of Florida
BSc in Biology - with Honors, 2008 - 2012
Truman State University
Determining the Pan-African sweet potato virome: understanding virus diversity, distribution and evolution and their impacts on sweet potato production in Africa
Network models portray the members of a microbial community along with inference about their interactions.
Root and the rhizosphere microbiomes associated with productive grafted tomato plants
Comparing fungal communities using a network approach.
Following are some of dirty little tricks (DLTs) in R that I have compiled through-out the years.
Current project… Exploratory analysis of rice varieties grown in Nepal.
If you wondering how to keep versions of your code, writing, or anything else… then here is a short guide that might quench your desire.
Steps to link R working directory with the remote server
Following are the list of courses that I participated as a TA, or an Instructor, or an Organizer:
PLP6905, Networks in Agricultural and Ecological Systems (TA)
Introduction to Network Models in R - Annual Meeting of American Phytopathological Society (2016)- (Instructor)
PLPTH 850, Introduction to R Programming Environment for Biologists (TA)
Using R for Applied Statistics- Hands on Training - (Organizer/ Instructor)
Protocol for tomato root homogenization pdf
RNA extraction using trizol, and cDNA preparation pdf
Cell counting using hemocytometer pdf
Cell Staining for Cancer Initiating Stem Cells (CISCs) pdf
Extracellular staining pdf
Staining of intracellular organelles using antibodies pdf
Preparing intermediate solution pdf
Preparing cell lysate ready for Western Blot pdf
Protocol for MTT assay pdf
Preparing lab media pdf
Preparing TBE solution for gel electrophoresis pdf